Internuclear Gene Silencing in Phytophthora infestans
نویسندگان
چکیده
منابع مشابه
Internuclear gene silencing in Phytophthora infestans.
Transformation of the diploid oomycete plant pathogen Phytophthora infestans with antisense, sense, and promoter-less constructs of the coding sequence of the elicitin gene inf1 resulted in transcriptional silencing of both the transgenes and the endogenous gene. Since heterokaryons obtained by somatic fusion of an inf1-silenced transgenic strain and a wild-type strain displayed stable gene sil...
متن کاملInternuclear gene silencing in Phytophthora infestans is established through chromatin remodelling.
In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other nuclei within heterokaryotic cells of the mycelium. Here we report experiments exploring the mechanism of internuclear gene silencing in P. infesta...
متن کاملCan silencing of transposons contribute to variation in effector gene expression in Phytophthora infestans?
Transposable elements are ubiquitous residents in eukaryotic genomes. Often considered to be genomic parasites, they can lead to dramatic changes in genome organization, gene expression, and gene evolution. The oomycete plant pathogen Phytophthora infestans has evolved a genome organization where core biology genes are predominantly located in genome regions that have relatively few resident tr...
متن کامل[Heteroplasmosis in Phytophthora infestans].
APCR and monozoospore plating was used to demonstrate a simultaneous presence of the mitochondria with mitochondrial DNA of haplotypes ILa and IIa in the mycelium of several Phytophthora infestans strains.
متن کاملPlant-mediated gene silencing restricts growth of the potato late blight pathogen Phytophthora infestans
Phytophthora infestans is an oomycete that causes severe damage to potato, and is well known for its ability to evolve rapidly in order to overcome resistant potato varieties. An RNA silencing strategy was evaluated here to clarify if small interfering RNA homologous to selected genes in P. infestans could be targeted from the plant host to reduce the magnitude of the infection. As a proof-of-c...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 1999
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80461-x